Bétous R, Mason AC, Rambo RP, Bansbach CE, Badu-Nkansah A, … Privacy, Help Careers. The stability and characteristics of a DNA Holliday junction switch 2020 Oct 26;21(21):7928. doi: 10.3390/ijms21217928. Anti-cancer immunotherapy using cancer-derived multiple epitope-peptides cocktail vaccination clinical studies in patients with refractory/persistent disease of uterine cervical cancer and ovarian cancer [phase 2]. In mammalian cells, HJs are processed by the BTR (BLM-topoisomerase IIIα-RMI1-RMI2) complex, the SLX-MUS (SLX1-SLX4-MUS81-EME1) complex, and the GEN1 resolvase. Knockdown of HJURP inhibits non-small cell lung cancer cell proliferation, migration, and invasion by repressing Wnt/beta-catenin signaling. Holliday junction (HJ) resolvases are necessary for the processing of persistent recombination intermediates before cell division. Identification of Holliday junction resolvases from humans and yeast. Garner E, Kim Y, Lach FP, Kottemann MC, Smogorzewska A. Yan J, Hong S, Guan Z, He W, Zhang D, Yin P. Nat Commun. Dual role of Nbs1 in the ataxia telangiectasia mutated-dependent DNA damage response. The shape, number, and distribution of chloroplast nucleoids change markedly depending on the cell cycle, developmental stage, and nutritional environment. DNA double-strand break repair-pathway choice in somatic mammalian cells. Overview of attention for article published in Genes & Development, January 2012. The kinetic analysis of Hjc-Holliday junction interaction indicated that mutations in the N-terminal segment greatly increased the dissociation rate constants of the Hjc-Holliday junction complex, explaining the decreased stability of the complex. This site needs JavaScript to work properly. Holliday junctions (HJs) are four-stranded DNA intermediates that arise during the recombinational repair of DNA double-strand breaks (DSBs). HJ … 2011 Mar 31;471(7340):642-6. doi: 10.1038/nature09790. COVID-19 is an emerging, rapidly evolving situation. Prevention and treatment information (HHS). Takeuchi S, Kagabu M, Shoji T, Nitta Y, Sugiyama T, Sato J, Nakamura Y. Oncoimmunology. De plus, j’ai pu mettre en évidence in vitro que HJURP facilitait la déposition du tétramère CENP-A/H4 sur de l’ADN satellite. Altmetric Badge. Their timely removal is crucial for faithful chromosome segregation and genome stability. FOIA Nature. Esc2 does not elicit these effects via stable interactions with substrates or their common SUMO E3. Homologous recombination is an essential process in maintaining genomic stability, and its defects can lead to serious human diseases, including cancer. Prevention and treatment information (HHS). First, mechanical stability of DNA origami structures is determined by the effective density of Holliday junctions along a particular stress direction. 2020 Mar 17;11(1):1417. doi: 10.1038/s41467-020-15242-8. The Holliday junction is resolved into two duplex DNA molecules via the action of junction resolving enzymes ... one can estimate the effect of magnesium ions on the stability of RSI, RTI and the open form, which are directly involved in the conformer transition (see Discussion). National Library of Medicine XerC is an efficient resolver of the recombination intermediate but a poor recombination initiator. 2015;80:103-9. doi: 10.1101/sqb.2015.80.027649. Thus, both methyl and hydroxymethyl modifications are accommodated without disrupting the structure or stability of the Holliday junction. SMARCAL1 catalyzes fork regression and Holliday junction migration to maintain genome stability during DNA replication Rémy Bétous , 1 Aaron C. Mason , 2 Robert P. Rambo , 3 Carol E. Bansbach , 1 Akosua Badu-Nkansah , 1 Bianca M. Sirbu , 1 Brandt F. Eichman , 1, 2 and David Cortez 1, 4 Genes Dev. We review these key advances and how they have cemented the status of HJ-processing enzymes as guardians of genome integrity and viability in mammalian cells. To cope with DNA damage encountered during genome duplication, a four-way (Holliday) junction is formed by joining two nearly identical DNA molecules by strand exchange (1–3). Cancers (Basel). Clipboard, Search History, and several other advanced features are temporarily unavailable. HJURP was considered a possible downstream target for ataxia telangiectasia mutated signaling, and its expression was increased by DNA double-strand breaks (DSB). FEBS J. Accessibility The structure is named after Robin Holliday, the molecular biologist who proposed its existence in 1964. Wen Y, Chen J, Li J, Arif W, Kalsotra A, Irudayaraj J. Toxicol Lett. Human GEN1 and the SLX4-associated nucleases MUS81 and SLX1 are essential for the resolution of replication-induced Holliday junctions. Solid tumours hijack the histone variant network. Waltes R, Kalb R, Gatei M, Kijas AW, Stumm M, Sobeck A, Wieland B, Varon R, Lerenthal Y, Lavin MF, Schindler D, Dörk T. Am J Hum Genet. Processing of homologous recombination intermediates is tightly coordinated to ensure that chromosomal integrity is maintained and tumorigenesis avoided. Epub 2015 Sep 14. 2021 Feb 10. doi: 10.1038/s41568-020-00330-0. Surprisingly, SMARCAL1 is capable of binding three-way and four-way Holliday junctions and model replication forks that lack a designed ssDNA region. This process, which requires the single-stranded DNA decatenation activity of topoisomerase III, is termed Holliday junction “dissolution” to distinguish it from Holliday junction “resolution” catalyzed by endonucleases (resolvases) that simply cleave the four-way junction. Jonction de Holliday — Une jonction de Holliday est formée lors de la recombinaison homologue entre deux molécules d'ADN portant la même information génétique (chromosomes homologues, chromatides sœurs, etc.). Epub 2009 Jul 26. 1, A (iv) and B]. The spatial control of GEN1 therefore contributes to genome stability, by avoiding competition with non-crossover promoting repair pathways. Holliday Junctions (4W-junctions) are highly conserved four-way DNA homologous replication junctions capable of undergoing salt-dependent conformational transitions between mobile open planar and immobile stacked-X forms (1). In contrast, during dissolution 26 , 151–162 (2012). Epub 2020 Apr 19. All-atom Molecular Dynamics (MD) simulations are reported on … It is also important in generating genetic diversity that is necessary for evolution. Please enable it to take advantage of the complete set of features! To cope with DNA damage encountered during genome duplication, a four-way (Holliday) junction is … Ip SC, Rass U, Blanco MG, Flynn HR, Skehel JM, West SC. Other_names [Holliday junction DNA helicase RuvA; Holliday junction ATP-dependent DNA helicase RuvA; ... Storage_stability Store at -20 degrees C. For long-term storage, store at -20 degrees C or -80 degrees C. Store working aliquots at 4 degrees C for up to one week. Holliday junctions (HJs) are key DNA intermediates in genetic recombination and are eliminated by nuclease, termed resolvase, to ensure genome stability. Esc2 is required for genome stability and binds to Holliday junctions and replication fork structures. Bétous, R. et al. Epub 2009 Apr 30. Cependant, le génome est continuellement soumis à des agressions provenant de facteurs exogènes tels que les radiations ionisantes, les ultraviolets, ou … This study investigates the effect of salt concentration on Holliday Junction conformation and dynamics. The DNA Holliday structure is a junction of four double helices, first proposed in 1964 [6] . HJURP was involved in the homologous recombination pathway in the DSB repair process through interaction with hMSH5 and NBS1, which is a part of the MRN protein complex. The Holliday junction is a four‐way DNA structure formed during the process of double strand break repair and DNA recombination in meiosis. DNA repair; HARP; Holliday junction; fork reversal; SIOD; SAXS; SMARCAL1 (SWI/SNF-related, matrix-associated, actin-dependent regulator of chromatin, subfamily A-like1), also known as DNA-dependent ATPase A and HARP (Hep-A-related protein), is a member of the SNF2 family of ATPases (Flaus et al. This suggests that there is an electronic effect of substituents at the pyrimidine Py position on the stability of four-stranded junctions. Inhibition of ATM reduced the level of HJURP protein, but not transcript, suggesting that HJURP is an ATM substrate and that phosphorylation stabilizes the HJURP protein. Search 15 grants from Pui Ho ... trinucleotide was identified as the core of the stable junction in this system The significance of this sequence motif has been confirmed by more recent studies on drug-bound and methylated junctions in this laboratory. GeneRIFs: Gene References Into Functions . 2008 Nov 20;456(7220):357-61. Furthermore, SMARCAL1 remodels these DNA substrates by promoting branch migration and fork regression. Nat Rev Cancer. Yen1/GEN1 are canonical Holliday junction resolvases that belong to the RAD2/XPG family. Our targeted screen found that Esc2 promotes the sumoylation of a Holliday junction dissolution complex and specific replisome proteins. HJURPwas considered a possible downstream target for ataxia telangiectasia mutated signaling, and its expression was increased by DNA double-strand breaks (DSB). HR is an essential process in both meiotic and mitotic cells. Spatial control of the GEN1 Holliday junction resolvase ensures genome stability. The kinetic analysis of Hjc-Holliday junction interaction indicated that mutations in the N-terminal segment greatly increased the dissociation rate constants of the Hjc-Holliday junction complex, explaining the decreased stability of the complex. 2020 Nov 11;9(1):1838189. doi: 10.1080/2162402X.2020.1838189. 2014 Jul;19(100):176-81. doi: 10.1016/j.dnarep.2014.03.013. Epub 2019 Aug 8. 2006;408:529-39. doi: 10.1016/S0076-6879(06)08033-5. Depending on the combination of cleavage orientations, which can be asymmetric or symmetric, this process can generate both crossover and noncrossover products. SMARCAL1 catalyzes fork regression and Holliday junction migration to maintain genome stability during DNA replication. Methods Enzymol. The single-crystal structures presented here, therefore, show how sequence affects the detailed geometry, and subsequently, the associated stability and conformational dynamics of the Holliday junction. This study investigates the effect of salt concentration on Holliday Junction conformation and dynamics. Recent studies have linked the deficiency of one or more of these enzymes to perturbed DNA replication, impaired crosslink repair, chromosomal instability, and defective mitoses, coupled with the transmission of widespread DNA damage and high levels of mortality. Nature. Elevated HJURP expression was associated with poor prognosis. DNA double-strand breaks can be repaired by homologous recombination, whose key intermediate is the four-way Holliday junction. SMARCAL1 catalyzes fork regression and Holliday junction migration to maintain genome stability during DNA replication. Holliday junctions (HJs) are four-stranded DNA intermediates that arise during the recombinational repair of DNA double-strand breaks (DSBs).
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